WebbTris-EDTA (TE), pH 8.0, BioUltra is a molecular biology (biotechnology) grade buffer that is DNase, RNase, phosphatase and protease free. TE buffer is useful as a general DNA or RNA extraction, suspension, washing, and storage buffer and in the preparation of nucleic acid for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain … WebbTE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5) is used to prepare the Quant-iT ™ PicoGreen dsDNA Reagent working solution, and to dilute the dsDNA standards and samples. Because the Quant-iT ™ PicoGreen dye is an extremely sensitive detection reagent for dsDNA, the TE solution used must be free of contaminating nucleic acids.
TE、pH 8.0、无 RNase
WebbWord lid of meld u aan om uw volgende vacature te zoeken. Solliciteer naar de functie van Micro-scale fluid handling scientist sample preparation CRYO-EM bij Thermo Fisher Scientific. Voornaam. Achternaam. E ... application of the protein containing buffer solution to the grid followed by blotting of the excess sample liquid and plunge freezing ... WebbSave time and simplify your buffer preparation step by using ready-made Fisher BioReagents 1X TE buffer solution; Eliminates the hassle of dilution or waiting for powder to dissolve; 1X solution contains 10mM tris and 1mM EDTA; Filtered through a 5 micron filter and autoclaved; Tested for the absence of DNase, RNase, and protease crs subjects
TE, pH 8.0, RNase-free - Thermo Fisher Scientific
WebbCulture tubes were spun in a centrifuge at 1,900 x g for 5 minutes, the pellet resuspended in 250 μL TE buffer, and then transferred into a tube containing 500 μL of 0.1 mm silica beads and subjected to three 30-second pulses with 30 seconds rest between pulses using a mini bead beater (BioSpec, USA). WebbFor each DNA fragment, a separate PCR reaction was performed in a 20 µl volume, containing 1.5 µl of the pCAMBIA plasmid template DNA, 2 µl of dNTP (2mM), 2 µl of Taq buffer (10x), 1.5 µl of MgCl2, 9.5 µl of PCR water (Thermo Fisher Scientific, USA), 3 µl of both forward and reverse primers (10 pmol each), and 0.5 µl of Taq DNA polymerase … Webb1. Equilibrate the 20X TE buffer, RiboGreen® reagent (200X concentrate and DEPC treated dH 2 O to room tempera-ture. Protect from light. 2. Prepare 1X TE with DEPC treated water. The volume needed depends on the total number of samples to be measured and the volume of RiboGreen working solution. 3. Dilute the RiboGreen® Dye stock in two ... build my 2022 corvette c8